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enzyme linked immunospot elispot assay  (Cellular Technology Ltd)


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    Structured Review

    Cellular Technology Ltd enzyme linked immunospot elispot assay
    In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed <t>by</t> <t>enzyme-linked</t> immunospot <t>(ELISpot)</t> assay. Data were shown as mean ± SD (n = 3).
    Enzyme Linked Immunospot Elispot Assay, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 96/100, based on 928 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/enzyme+linked+immunospot+assay+elispot/Human+IFN-%CE%B3+Single-Color+ELISPOT/pmc12926576-337-88-89
    Average 96 stars, based on 928 article reviews
    enzyme linked immunospot elispot assay - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy"

    Article Title: Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.02.018

    In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay. Data were shown as mean ± SD (n = 3).
    Figure Legend Snippet: In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay. Data were shown as mean ± SD (n = 3).

    Techniques Used: In Vivo, Activation Assay, Injection, Flow Cytometry, Enzyme-linked Immunospot

    Related Articles

    Enzyme-linked Immunospot:

    Article Title: Comparative Outcomes and Immune Mechanisms in Murine Endothelial Versus Penetrating Keratoplasty
    Article Snippet: Methods: Allogeneic EK and PK were performed in BALB/c recipient mice using fully disparate C57BL/6 donors; syngeneic EK recipients served as controls.. Graft clarity was monitored over 16 weeks by slitlamp biomicroscopy and scored using standardized opacity grading.. Anterior segment optical coherence tomography (AS-OCT) was used to measure central corneal thickness.

    Article Title: Rapid and potent induction of protective mucosal immunity against SARS-CoV-2 by an adenovirus-vectored nanoparticle vaccine.
    Article Snippet: Data were analyzed using FlowJo v10.8.1. .. Enzyme-Linked Immunospot Assay (ELISpot) IFN-γ ELISpot: Plates were coated with an anti-mouse IFN-γ capture antibody overnight. ..

    Article Title: Combination mRNA Vaccine Adjuvanted with CpG Oligodeoxynucleotides Enhances Protection against Respiratory Virus Infection.
    Article Snippet: .. Enzyme-Linked Immunospot Assay (ELISPOT) Mice were euthanized humanely, and their spleens harvested and mechanically dissociated in chilled 1640 medium (BasalMedia, Shanghai) supplemented with 2% fetal bovine serum (FBS) (ExCell Bio) to obtain single-cell suspensions. .. The cell pellet was then treated with 10 mL of cold red blood cell lysis buffer (Solarbio) for 10 min at room temperature to remove red blood cells.

    Article Title: Methods and compositions for cardiovascular disease detection and management
    Article Snippet: .. Enzyme-Linked Immunospot Assay (ELISPOT) is an immunoassay that can detect an antibody specific for a protein or antigen. ..

    Article Title: Cancer neoantigens and their utilities in cancer vaccines and TCR-based cancer immunotherapy
    Article Snippet: .. Enzyme-Linked Immunospot Assay (ELISpot) is an immunoassay that can detect an antibody specific for a protein or antigen. ..

    Article Title: The latent tuberculosis infection survey using two interferon γ release assay tests among the elderly in a well-confined rural county in Eastern China.
    Article Snippet: The first is the QuantiFERONTB Gold In-Tube test (QFT-IT), developed by Cellestis in Australia, which employed enzyme-linked immunosorbent assay (ELISA) to directly measure the concentration of interferon-γ in whole blood. .. The other was the T-cell spot test for TB (T-SPOT.TB), produced by Oxford Immunotec in the UK utilizing enzyme-linked immunospot assay (ELISPOT) to quantify the number of spots representing MTB-specific effector T cells [13]. ..

    Article Title: Ferritin-based hybrid protein particle vaccine combining M2e antigen of influenza A virus and S-protein tandem epitopes of SARS-CoV-2.
    Article Snippet: Ferritin functions as an antigen delivery platform for enhancing immune responses and is widely employed in vaccine development.. Here, we propose a ferritin-based hybrid protein particle strategy using Escherichia coli to produce a hybrid protein particle vaccine combining an M2e antigen of influenza A and an S-protein tandem epitopes (STE antigen) of SARS-CoV-2.. M2e and STE antigens were independently fused to the N-terminus of the human ferritin heavy chain (FTH), and the two expression cassettes were placed within a single open reading frame under the control of the same promoter in the pET-30a vector.

    Article Title: The latent tuberculosis infection survey using two interferon γ release assay tests among the elderly in a well-confined rural county in Eastern China
    Article Snippet: The first is the QuantiFERON-TB Gold In-Tube test (QFT-IT), developed by Cellestis in Australia, which employed enzyme-linked immunosorbent assay (ELISA) to directly measure the concentration of interferon-γ in whole blood. .. The other was the T-cell spot test for TB (T-SPOT.TB), produced by Oxford Immunotec in the UK utilizing enzyme-linked immunospot assay (ELISPOT) to quantify the number of spots representing MTB-specific effector T cells [ ]. ..

    Mouse Assay:

    Article Title: Combination mRNA Vaccine Adjuvanted with CpG Oligodeoxynucleotides Enhances Protection against Respiratory Virus Infection.
    Article Snippet: .. Enzyme-Linked Immunospot Assay (ELISPOT) Mice were euthanized humanely, and their spleens harvested and mechanically dissociated in chilled 1640 medium (BasalMedia, Shanghai) supplemented with 2% fetal bovine serum (FBS) (ExCell Bio) to obtain single-cell suspensions. .. The cell pellet was then treated with 10 mL of cold red blood cell lysis buffer (Solarbio) for 10 min at room temperature to remove red blood cells.

    Single Cell:

    Article Title: Combination mRNA Vaccine Adjuvanted with CpG Oligodeoxynucleotides Enhances Protection against Respiratory Virus Infection.
    Article Snippet: .. Enzyme-Linked Immunospot Assay (ELISPOT) Mice were euthanized humanely, and their spleens harvested and mechanically dissociated in chilled 1640 medium (BasalMedia, Shanghai) supplemented with 2% fetal bovine serum (FBS) (ExCell Bio) to obtain single-cell suspensions. .. The cell pellet was then treated with 10 mL of cold red blood cell lysis buffer (Solarbio) for 10 min at room temperature to remove red blood cells.

    Spot Test:

    Article Title: The latent tuberculosis infection survey using two interferon γ release assay tests among the elderly in a well-confined rural county in Eastern China.
    Article Snippet: The first is the QuantiFERONTB Gold In-Tube test (QFT-IT), developed by Cellestis in Australia, which employed enzyme-linked immunosorbent assay (ELISA) to directly measure the concentration of interferon-γ in whole blood. .. The other was the T-cell spot test for TB (T-SPOT.TB), produced by Oxford Immunotec in the UK utilizing enzyme-linked immunospot assay (ELISPOT) to quantify the number of spots representing MTB-specific effector T cells [13]. ..

    Article Title: The latent tuberculosis infection survey using two interferon γ release assay tests among the elderly in a well-confined rural county in Eastern China
    Article Snippet: The first is the QuantiFERON-TB Gold In-Tube test (QFT-IT), developed by Cellestis in Australia, which employed enzyme-linked immunosorbent assay (ELISA) to directly measure the concentration of interferon-γ in whole blood. .. The other was the T-cell spot test for TB (T-SPOT.TB), produced by Oxford Immunotec in the UK utilizing enzyme-linked immunospot assay (ELISPOT) to quantify the number of spots representing MTB-specific effector T cells [ ]. ..

    Produced:

    Article Title: The latent tuberculosis infection survey using two interferon γ release assay tests among the elderly in a well-confined rural county in Eastern China.
    Article Snippet: The first is the QuantiFERONTB Gold In-Tube test (QFT-IT), developed by Cellestis in Australia, which employed enzyme-linked immunosorbent assay (ELISA) to directly measure the concentration of interferon-γ in whole blood. .. The other was the T-cell spot test for TB (T-SPOT.TB), produced by Oxford Immunotec in the UK utilizing enzyme-linked immunospot assay (ELISPOT) to quantify the number of spots representing MTB-specific effector T cells [13]. ..

    Article Title: The latent tuberculosis infection survey using two interferon γ release assay tests among the elderly in a well-confined rural county in Eastern China
    Article Snippet: The first is the QuantiFERON-TB Gold In-Tube test (QFT-IT), developed by Cellestis in Australia, which employed enzyme-linked immunosorbent assay (ELISA) to directly measure the concentration of interferon-γ in whole blood. .. The other was the T-cell spot test for TB (T-SPOT.TB), produced by Oxford Immunotec in the UK utilizing enzyme-linked immunospot assay (ELISPOT) to quantify the number of spots representing MTB-specific effector T cells [ ]. ..



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    Image Search Results


    In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay. Data were shown as mean ± SD (n = 3).

    Journal: Bioactive Materials

    Article Title: Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy

    doi: 10.1016/j.bioactmat.2026.02.018

    Figure Lengend Snippet: In vivo DC maturation and T cell activation in C57BL/6J mice induced by mOVA/H 18 NPs through intravenous injection. C57BL/6J mice were vaccinated with different formulations on Day 0 and Day 5. On Day 10, the spleens of mice were collected and analyzed by flow cytometry. Quantification analysis of (A) CD80 + CD86 + DCs and (B) CD40 + DCs in the spleen. Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay. Data were shown as mean ± SD (n = 3).

    Article Snippet: Quantification analysis of (C) CD3 + CD4 + T cells and (D) CD3 + CD8 + T cells in the spleen. (E) Quantification analysis and (F) representative flow cytometry contour plots of OVA-specific CD8 + T cells among all cell populations in the spleen. (G) Quantification analysis and (H) representative flow cytometry contour plots of IFN-γ + CD8 + T cells among all cell populations in the spleen. (I) Quantification results and (J) representative images of IFN- γ -secreting immune cells in the spleen of mice analyzed by enzyme-linked immunospot (ELISpot) assay.

    Techniques: In Vivo, Activation Assay, Injection, Flow Cytometry, Enzyme-linked Immunospot